Electrochemical control of the enzymatic polymerization of PEG hydrogels: formation of spatially controlled biological microenvironments

Vincent Milleret; Benjamin R Simona; Philipp S Lienemann; János Vörös; Martin Ehrbar

doi:10.1002/adhm.201300479

Electrochemical control of the enzymatic polymerization of PEG hydrogels: formation of spatially controlled biological microenvironments

Adv Healthc Mater. 2014 Apr;3(4):508-14. doi: 10.1002/adhm.201300479. Epub 2013 Oct 30.

Authors

Vincent Milleret¹, Benjamin R Simona, Philipp S Lienemann, János Vörös, Martin Ehrbar

Affiliation

¹ Laboratory for Cell and Tissue Engineering, Department of Obstetrics, University Hospital Zurich, Schmelzbergstrasse 12, PATH G 48b, 8091, Zurich, Switzerland.

PMID: 24574303
DOI: 10.1002/adhm.201300479

Abstract

Control of pH gradient profile at the electrode-electrolyte interfaces allows the control of the enzymatic PEG-hydrogel polymerization. By tuning the solution pH, buffer capacity, and the applied current, the extent of the local inhibition and confinement of the Factor XIII-mediated polymerization of PEG are controlled. This technology opens new perspectives for the production of 3D-structured biological microenvironments.

Keywords: PEG-hydrogels; electrochemistry; microenvironment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemistry*
Biocompatible Materials / metabolism
Cell Engineering / methods*
Cell Line
Cellular Microenvironment
Dimethylpolysiloxanes / chemistry
Electrochemical Techniques / methods*
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
Hydrogel, Polyethylene Glycol Dimethacrylate / metabolism
Hydrogen-Ion Concentration
Polyethylene Glycols / chemistry*
Polyethylene Glycols / metabolism
Polymerization

Substances

Biocompatible Materials
Dimethylpolysiloxanes
Hydrogel, Polyethylene Glycol Dimethacrylate
Polyethylene Glycols
baysilon