A total of eight mining subsidence waters, including five sites in Huainan "Panxie" Mining Areas (PXS-1, PXS-2, PXS-3, PXS-4, and PXS-5) and three sites in Huaibei "Zhu-Yang huang" Mining Areas (HBDH, HBZH, HBNH), were selected to study the nutrient temporal and spatial distribution and trophic states. Among the sites, three sites (PXS-1, PXS-3, and HBDH) showed higher nutrient level and could be classified into moderate eutrophication, whereas the other five were in moderate nutrient level and mild eutrophication. Overall, the nutrient level of Huainan mining subsidence waters was higher than that of Huaibei mining subsidence waters. All the test samples in the two mining areas had a higher ratio of nitrogen to phosphorus (N:P), being 25-117 in Huainan and 17-157 in Huaibei, and with a seasonal variety, the lowest in growth season. The dissolved inorganic phosphorus (DIP) in total phosphorous (TP) occupied a small percentage, being averagely 15.4% and 18.4% in Huainan and Huaibei mining areas, respectively. Nitrate was the main specie of dissolved inorganic nitrogen (DIN), with the ratio of nitrate to DIN being 74% and 89% in Huainan and Huaibei mining areas, respectively. Relative to the waters age, human activities could be one of the main factors responsible for the high nutrient level and the faster eutrophication process of these waters.