CTCF-mediated reduction of vigilin binding affects the binding of HP1α to the satellite 2 locus

FEBS Lett. 2014 May 2;588(9):1549-55. doi: 10.1016/j.febslet.2014.02.013. Epub 2014 Feb 20.

Abstract

CCCTC-binding factor (CTCF) has been implicated in numerous aspects of chromosome biology, and vigilin, a multi-KH-domain protein, participates in heterochromatin formation and chromosome segregation. We previously showed that CTCF interacts with vigilin. Here, we show that human vigilin, but not CTCF, colocalizes with HP1α on heterochromatic satellite 2 and β-satellite repeats. CTCF up-regulates the transcription of satellite 2, while vigilin down-regulates it. Vigilin depletion or CTCF overexpression reduces the binding of HP1α on the satellite 2 locus. Furthermore, overexpression of CTCF resists the loading of vigilin onto the satellite 2 locus. Thus CTCF may regulate vigilin behavior and thus indirectly influence the binding of HP1α to the satellite 2 locus.

Keywords: CCCTC-binding factor; HP1α; Satellite 2; Vigilin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCCTC-Binding Factor
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / metabolism*
  • DNA, Satellite / metabolism*
  • HEK293 Cells
  • HeLa Cells
  • Heterochromatin / metabolism
  • Humans
  • MCF-7 Cells
  • Protein Binding
  • Protein Transport
  • RNA-Binding Proteins / metabolism*
  • Repressor Proteins / physiology*
  • Transcription, Genetic
  • Transcriptional Activation

Substances

  • CBX5 protein, human
  • CCCTC-Binding Factor
  • CTCF protein, human
  • Chromosomal Proteins, Non-Histone
  • DNA, Satellite
  • Heterochromatin
  • RNA-Binding Proteins
  • Repressor Proteins
  • Chromobox Protein Homolog 5
  • high density lipoprotein binding protein