Sublingual administration of a helper-dependent adenoviral vector expressing the codon-optimized soluble fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in mice

Antiviral Res. 2014 May:105:72-9. doi: 10.1016/j.antiviral.2014.02.003. Epub 2014 Feb 19.

Abstract

Sublingual (s.l.) immunization has been described as a convenient and safe way to induce mucosal immune responses in the respiratory and genital tracts. We constructed a helper-dependent adenoviral (HDAd) vector expressing a condon-optimized soluble fusion glycoprotein (sFsyn) of respiratory syncytial virus (HDAd-sFsyn) and explored the potential of s.l. immunization with HDAd-sFsyn to stimulate immune responses in the respiratory mucosa. The RSV specific systemic and mucosal immune responses were generated in BALB/c mice, and the serum IgG with neutralizing activity was significantly elevated after homologous boost with s.l. application of HDAd-sFsyn. Humoral immune responses could be measured even 14weeks after a single immunization. Upon challenge, s.l. immunization with HDAd-sFsyn displayed an effective protection against RSV infection. These findings suggest that s.l. administration of HDAd-sFsyn acts as an effective and safe mucosal vaccine against RSV infection, and may be a useful tool in the prevention of RSV infection.

Keywords: Human respiratory syncytial virus; Immune responses; Soluble fusion glycoprotein; Sublingual immunization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Administration, Sublingual
  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood
  • Disease Models, Animal
  • Drug Carriers / administration & dosage*
  • Female
  • Immunity, Mucosal
  • Immunoglobulin G / blood
  • Mice, Inbred BALB C
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / prevention & control*
  • Respiratory Syncytial Virus Vaccines / administration & dosage*
  • Respiratory Syncytial Virus Vaccines / genetics
  • Respiratory Syncytial Virus Vaccines / immunology*
  • Respiratory Syncytial Virus, Human / genetics
  • Respiratory Syncytial Virus, Human / immunology*
  • Serum / immunology
  • Vaccines, Synthetic / administration & dosage

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Drug Carriers
  • Immunoglobulin G
  • Respiratory Syncytial Virus Vaccines
  • Vaccines, Synthetic