Objective: To investigate the optimal technology of extraction of fufang zhenqin feining capsules and establish a method for determination its oleanolic acid.
Methods: Orthogonal test was employed for selecting the optimum extraction technology by the index of the content of oleanolic acid in extraction. HPLC was performed on a C18 (250 mm x 4.6 mm, 5 microm) column using methanol-0.3% triethylamine solution (80: 20) as the mobile phase at a flow rate of 1 ml/min. The detection wavelength was set at 210 nm. The column temperature was 30 degrees C and the injection size was 25 microL.
Results: The optimum extraction technology was as follows: the concertration of alcohol was 70%, the ratio of solid/material was 8: 1, extracting 3 times with half an hour for each time. The linear range of oleanolic acid was 59.68 - 596.8 microg/ml (r = 0.9997), the average recovery was 99.79% (RSD = 0.5%).
Conclusion: The considerable extraction rate of active components in the drugs is achieved by applying the selected technology, and the simple method is fit for production. The established HPLC method is simple, accurate, special and can be used for the quality control of fufang zhenqin feining capsules.