A study on Anoboeno strains was carried out to investigate the effect of combined stress of polyethylene glycol 6000 (0,-5 bar and -7 bar) an d temperature (30 degreeC and 45 degreeC) onphotosynthetic pigments (chlorophyll a, carotenoids, phycobilins) and total proteins as stress metabolites. The selected strains, A. oryzoe and A. ellipsosporo were sensitive to osmotic stress at ambient temperature of 30 degreeC and increase in the temperature to 45 degreeC was harmful to the growth of Anoboeno strains. Chlorophyll a contents decreased at 30 degreeC and -7 bar pressure from 8.868 to 0.710 microg ml-1 and 4.360 to 0.220 microg ml-1 in A. oryzae and in A. ellipsospora, respectively and at -7 bar osmotic stress and 45 microC temperature, decrease in Chi a content of A.oryzae was 92.9%, however A. ellipsospora was highly sensitive and could not survive under these conditions. Carotenoids and phycobilins also showed decreasing trends with increase in temperature and osmotic potential. Moreover, combined stress adversely depleted the cellular activities leading to a marked decrease in total protein contents of the cell. A.oryzae and A.ellipsospora showed varying tolerance potential to osmotic and temperature stresses. The results indicated that A. ellipsospora was more sensitive towards these stresses in comparison to A.oryzoe.