The role of noncoding regions of classical swine fever virus C-strain in its adaptation to the rabbit

Virus Res. 2014 Apr:183:117-22. doi: 10.1016/j.virusres.2014.02.003. Epub 2014 Feb 16.

Abstract

Classical swine fever (CSF) is a devastating disease of swine caused by classical swine fever virus (CSFV). C-strain, a modified live vaccine against CSF, was developed through hundreds of passages of a highly virulent CSFV in the rabbit in China in the mid-1950s. To identify the role of noncoding regions (NCRs) of C-strain in its adaptation to the rabbit, we generated and evaluated a series of chimeric viruses derived from C-strain and the highly virulent Shimen strain. The results demonstrated that the NCRs of the C-strain were essential for its fever induction in rabbits and the coding region but not NCRs was necessary for its replication in the spleen of rabbits.

Keywords: Adaptation to the rabbit; C-strain; Classical swine fever virus; Fever induction; Noncoding regions; Replication in the spleen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological*
  • Animals
  • China
  • Classical Swine Fever Virus / genetics*
  • Classical Swine Fever Virus / physiology*
  • RNA, Untranslated / genetics*
  • RNA, Viral / genetics*
  • Rabbits
  • Recombination, Genetic
  • Serial Passage
  • Spleen / virology
  • Virus Replication*

Substances

  • RNA, Untranslated
  • RNA, Viral