Abstract
Pairs of monoclonal antibodies (mAb) defining epitopes T 11.1 and T 11.2 on the CD 2 molecule are mitogenic for purified human T cells in the presence of a submitogenic dose of 12-O-tetradecanoylphorbol 13-acetate (TPA). Anti-CD 28 mAb can substitute for the action of TPA in the anti-CD 2-induced proliferative response of resting T cells, whereas each signal alone is unable to mediate this effect. Co-stimulation by anti-CD 2 plus anti-CD 28 mAb is monocyte independent and besides resting T cells also induces strong proliferation of thymocytes and pre-activated T cells. Modulation of the CD 3-T cell receptor complex does not inhibit the co-stimulatory effects of anti-CD 2 plus anti-CD 28 mAb. The effect is largely dependent on endogenously produced interleukin 2, since the response is strongly inhibited in the presence of mAb against the 55-kDa interleukin 2 receptor chain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation / immunology
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Antigens, Differentiation / physiology*
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Antigens, Differentiation, T-Lymphocyte / immunology
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Antigens, Differentiation, T-Lymphocyte / physiology*
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CD2 Antigens
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CD28 Antigens
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CD3 Complex
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Epitopes / immunology
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Humans
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Interleukin-2 / physiology
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Lymphocyte Activation* / drug effects
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Receptors, Antigen, T-Cell / physiology
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Receptors, Immunologic / immunology
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Receptors, Immunologic / physiology*
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Receptors, Interleukin-2
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation
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Antigens, Differentiation, T-Lymphocyte
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CD2 Antigens
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CD28 Antigens
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CD3 Complex
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Epitopes
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Interleukin-2
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Receptors, Antigen, T-Cell
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Receptors, Immunologic
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Receptors, Interleukin-2
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Tetradecanoylphorbol Acetate