Wheatstone bridge giant-magnetoresistance based cell counter

Chiun-Peng Lee; Mei-Feng Lai; Hao-Ting Huang; Chi-Wen Lin; Zung-Hang Wei

doi:10.1016/j.bios.2014.01.028

Wheatstone bridge giant-magnetoresistance based cell counter

Biosens Bioelectron. 2014 Jul 15:57:48-53. doi: 10.1016/j.bios.2014.01.028. Epub 2014 Jan 25.

Authors

Chiun-Peng Lee¹, Mei-Feng Lai², Hao-Ting Huang¹, Chi-Wen Lin², Zung-Hang Wei³

Affiliations

¹ Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan ROC.
² Institute of Nanoengineering and Microsystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan ROC.
³ Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan ROC. Electronic address: wei@pme.nthu.edu.tw.

PMID: 24534580
DOI: 10.1016/j.bios.2014.01.028

Abstract

A Wheatstone bridge giant magnetoresistance (GMR) biosensor was proposed here for the detection and counting of magnetic cells. The biosensor was made of a top-pinned spin-valve layer structure, and it was integrated with a microchannel possessing the function of hydrodynamic focusing that allowed the cells to flow in series one by one and ensured the accuracy of detection. Through measuring the magnetoresistance variation caused by the stray field of the magnetic cells that flowed through the microchannel above the GMR biosensor, we can not only detect and count the cells but we can also recognize cells with different magnetic moments. In addition, a magnetic field gradient was applied for the separation of different cells into different channels.

Keywords: Biosensor; Cell counting; Cell separation; Giant magnetoresistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques / instrumentation
Cell Count / instrumentation*
Cell Line
Cell Line, Tumor
Cell Separation / instrumentation
Equipment Design
Humans
Hydrodynamics
Magnetics / instrumentation*
Mice