Limited research exists on rest-pause or cluster-set (CS) protocols. Acute effects of a traditional set (TS) and CS protocols of resistance exercise on serum growth hormone (GH), cortisol (C), blood lactate (BL), countermovement vertical jump (CMVJ) and standing long jump (SLJ) were compared. Eleven resistance-trained males (22.9 ± 2.6 year; 176.9 ± 10.6 cm; 78.5 ± 1.6 kg; 12.9 ± 3.1% BF) completed one repetition maximum tests for clean pull (CP), back squat (BS) and bench press (BP). Subjects were then randomly assigned to TS or CS protocols for sessions 2 and 3, and performed CP and BS lifts followed by two circuits of three sets of three exercises. GH, C, BL, CMVJ and SLJ were measured pre-exercise (Pre), mid-exercise following completion of CS or TS protocol (Mid), immediately (IP), 15 (15P) and 30 (30P) minutes post-exercise. Repeated measures ANOVAs examined differences in GH, C, BL, CMVJ and SLJ. No differences (p>0.05) existed between protocols for GH and C. GH levels 15P were elevated (p<0.05) above 30P (15.78 + 4.66 vs. 12.10 + 4.66 µg·L(-1)). C levels 30P were elevated (p<0.05) above Pre (716.85 + 102.56 vs. 524.79 + 75.79 nmol·L(-1)). Interaction (p <0.05) existed between protocol and time for BL; mid-BL was lower for CS than TS (7.69 ± 3.73 vs. 12.78 ± 1.90 mmol·L(-1)). Pooled data for CMVJ and SLJ were greater (p <0.05) across the CS protocol. The less metabolically taxing CS protocol resulted in better sustainability of jump measures.