Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors

Dalton Trans. 2014 Apr 14;43(14):5265-8. doi: 10.1039/c4dt00356j.

Abstract

Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flavin Mononucleotide / chemistry*
  • Fluorescence Resonance Energy Transfer
  • Metal Nanoparticles / chemistry
  • Metals, Rare Earth / chemistry*
  • Oxidation-Reduction
  • Oxidoreductases / chemistry*

Substances

  • Metals, Rare Earth
  • Flavin Mononucleotide
  • Oxidoreductases
  • pentaerythritol tetranitrate reductase