Coffea arabica instant coffee--chemical view and immunomodulating properties

Carbohydr Polym. 2014 Mar 15:103:418-26. doi: 10.1016/j.carbpol.2013.12.068. Epub 2014 Jan 3.

Abstract

Results of chemical analyses and immunological studies of two Coffea arabica instant coffee powders obtained by freeze-dried (ICPf) and spray-dried (ICPs) procedures, and arabinogalactan-protein (AGP3) obtained from ICPf are presented. For instant coffee powders no significant differences have been found in carbohydrate (ICPf: 37%, ICPs: 38%) as well as in caffeine (ICPf: 3.0%, ICPs: 3.4%) contents. Their (1)H NMR spectra revealed differences in trigonelline and chlorogenic acids content and in a degree of AGP backbone substitution. Immunobiological tests of all samples (ICPf, ICPs, AGP2 and AGP3) revealed a significant immunostimulatory effect on induction of interleukin 2 and free radicals secretion by mice immunocytes. Moreover, tests revealed more pronounced effect of arabinogalactans AGP2 and AGP3 compared to instant coffee powders (ICPf and ICPs).

Keywords: Arabinogalactan-protein; Coffea arabica beans; FT-IR; Immunobiological activity; Instant coffee powder; NMR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Cells, Cultured
  • Coffea / chemistry*
  • Female
  • Free Radicals / immunology
  • Immunologic Tests
  • Interleukin-2 / immunology
  • Mice
  • Mice, Inbred BALB C
  • Spleen / cytology
  • Spleen / drug effects*
  • Spleen / immunology

Substances

  • Adjuvants, Immunologic
  • Free Radicals
  • Interleukin-2