Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2-immobilized titanium via click reaction

Carbohydr Polym. 2014 Mar 15:103:170-8. doi: 10.1016/j.carbpol.2013.12.023. Epub 2013 Dec 16.

Abstract

In the present study, we report about the efficacy of titanium surface-immobilized with bone morphogenetic protein-2 (BMP-2) via click reaction on enhanced osteogenic differentiation of MC3T3-E1 cells. The surface was characterized by static contact angles and XPS measurements, which indicated that pristine titanium (Ti-1) was successfully surface-modified via click chemistry (aminated titanium, Ti-4). By quantitative analysis of heparin immobilized on aminated titanium (Ti-4), we found that the Ti-4 can be used as a good candidate to immobilize biomolecules such as heparin. BMP-2 from titanium immobilized with BMP-2 (Ti-6) was released for a period of 28 days in a sustained manner. The highest proliferation rate of MC3T3-E1 cells was observed on Ti-6. Through in vitro tests including alkaline phosphatase (ALP) activity, calcium deposition and real-time polymerase chain reaction (real-time PCR), we found that Ti-6 can be used as a good implant to enhance the osteogenic differentiation of MC3T3-E1 cells.

Keywords: BMP-2; MC3T3-E1 cell; Osteogenic differentiation; Titanium surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Bone Morphogenetic Protein 2 / chemistry*
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Click Chemistry
  • Mice
  • Osteogenesis / drug effects*
  • Recombinant Proteins / chemistry*
  • Structure-Activity Relationship
  • Surface Properties
  • Titanium / chemistry
  • Titanium / pharmacology*

Substances

  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Recombinant Proteins
  • Titanium