Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification

Cheng-Yi Hong; Xian Chen; Juan Li; Jing-Hua Chen; Guonan Chen; Huang-Hao Yang

doi:10.1039/c3cc48996e

Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification

Chem Commun (Camb). 2014 Mar 28;50(25):3292-5. doi: 10.1039/c3cc48996e. Epub 2014 Feb 14.

Authors

Cheng-Yi Hong¹, Xian Chen, Juan Li, Jing-Hua Chen, Guonan Chen, Huang-Hao Yang

Affiliation

¹ The Key Lab of Analysis and Detection for Food Safety of MOE and Fujian Provincial, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China. hhyang@fio.org.cn.

PMID: 24525625
DOI: 10.1039/c3cc48996e

Abstract

We have developed a simple method for direct detection of circulating microRNAs in serum by using the p19 protein-functionalized magnetic beads (PFMBs) for specific enrichment and rolling circle amplification (RCA). The detection limit for microRNAs is 1 fM. Therefore, the proposed method has the potential of being used in the analysis of circulating microRNAs and clinical diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Magnetic Phenomena
MicroRNAs / blood*
MicroRNAs / chemistry
Neoplasms / blood*
Nucleic Acid Amplification Techniques / methods*
Viral Proteins / chemistry

Substances

MicroRNAs
Viral Proteins