Preparation and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT)

Curr Protoc Stem Cell Biol. 2013 Nov 13:27:2B.5.1-2B.5.12. doi: 10.1002/9780470151808.sc02b05s27.

Abstract

In this unit, previously described methods are expanded upon, where procedures relating to the preparation, culturing, and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT) are outlined. Not only is a detailed methodology of how to engineer such spheroids are presented, but a full account of how to induce and analyze osteogenesis in these ASC-MT constructs is given along with relevant figures to help better illustrate the methods described.

Keywords: 3D-culture; adipose-derived stromal cells; osteogenesis.

MeSH terms

  • Adipose Tissue / cytology*
  • Animals
  • Cell Differentiation*
  • Cell Proliferation
  • Cell Separation
  • Cells, Cultured
  • Male
  • Mice
  • Osteogenesis*
  • Spheroids, Cellular / cytology*
  • Stromal Cells / cytology
  • Tissue Culture Techniques / methods*
  • Tissue Scaffolds*