Excretory/secretory (E/S) antigen of adult Fasciola gigantica was used for diagnosis of fasciolosis in cattle and buffaloes sera from slaughter houses and different places in and around Chennai, Tamil Nadu. A total of 6.4 mg of E/S antigen was obtained from the 60 flukes collected from slaughter houses. Coprological examination was done to detect endoparasitic infection in 321 fecal samples. Dot-enzyme immuno assay (Dot-EIA) was standardized with optimum working dilution of E/S antigen, serum, and conjugate by the checker board titration as 1 in 10 (50 ng/ml), 1 in 20, and 1 in 1,000, respectively. A total of 321 sera samples consisting of 168 cattle and 153 buffaloes were tested by Dot-EIA. A total of 25 cattle (14.88 %) and 38 buffaloes (24.83 %) showed positive results in Dot-EIA. Sensitivity of Dot-EIA for cattle and buffaloes sera was 100 %. Specificity of Dot-EIA for cattle and buffaloes was 95.28 and 92.10 %, respectively in comparison to fecal examination by sedimentation method. Dot-EIA can be easily adapted in the field to detect fasciolosis in cattle and buffaloes.
Keywords: Buffaloes; Cattle; Dot-enzyme immuno assay; Fasciola gigantica.