Neuroprotective effect of the fermented Gumiganghwal-tang

Bo-Ra Yun; Jin Bae Weon; Jiwoo Lee; Min Rye Eom; Choong Je Ma

doi:10.1016/j.jbiosc.2014.01.004

Neuroprotective effect of the fermented Gumiganghwal-tang

J Biosci Bioeng. 2014 Aug;118(2):235-8. doi: 10.1016/j.jbiosc.2014.01.004. Epub 2014 Feb 4.

Authors

Bo-Ra Yun¹, Jin Bae Weon¹, Jiwoo Lee¹, Min Rye Eom¹, Choong Je Ma²

Affiliations

¹ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.
² Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: cjma@kangwon.ac.kr.

PMID: 24503423
DOI: 10.1016/j.jbiosc.2014.01.004

Abstract

Gumiganghwal-tang (GT) is a traditional herbal prescription widely used to treat inflammatory diseases in Asia. In this study, we evaluated neuroprotective effect and acetylcholinesterase (AChE) inhibitory activity of GT and compared with fermented GT (FGT). In order to better understand the neuroprotective mechanism, intracellular reactive oxygen species (ROS) production was investigated and high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis of contents of GT and FGT were conducted. As a result, FGT showed more potent protective effect against glutamate-induced HT22 cell death than GT and inhibited AChE activity. The neuroprotective effect of FGT is associated with inhibition of ROS production. The HPLC-DAD analytical results indicated that FGT contains higher content of bergapten and atractylenolide III than that of GT. In conclusion, FGT have neuroprotective effect and AChE inhibition, and may be useful source for treatment of neurodegenerative disease.

Keywords: Antioxidant; Fermentation; Gumiganghwal-tang; Herb; Neuroprotection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylcholinesterase / chemistry
Animals
Apoptosis
Cell Death / drug effects
Cell Line
Cholinesterase Inhibitors / chemistry
Cholinesterase Inhibitors / metabolism
Cholinesterase Inhibitors / pharmacology
Drug Evaluation, Preclinical
Fermentation
Lactobacillus / metabolism
Medicine, Korean Traditional
Mice
Neurodegenerative Diseases / drug therapy
Neuroprotective Agents / chemistry
Neuroprotective Agents / metabolism
Neuroprotective Agents / pharmacology*
Plant Extracts / chemistry
Plant Extracts / metabolism
Plant Extracts / pharmacology*
Reactive Oxygen Species / metabolism

Substances

Cholinesterase Inhibitors
Neuroprotective Agents
Plant Extracts
Reactive Oxygen Species
kumi-kyokatsu-to
Acetylcholinesterase