Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate

Kaori Tsutsumi; Nagahito Saito; Yumi Kawazoe; Hong-Kean Ooi; Toshikazu Shiba

doi:10.1371/journal.pone.0086834

Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate

PLoS One. 2014 Feb 3;9(2):e86834. doi: 10.1371/journal.pone.0086834. eCollection 2014.

Authors

Kaori Tsutsumi¹, Nagahito Saito², Yumi Kawazoe³, Hong-Kean Ooi⁴, Toshikazu Shiba⁵

Affiliations

¹ Department of Biological Sciences and Engineering, Faculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
² Department of Internal Medicine, Gastroenterology & Hematology Section, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan.
³ Regenetiss Inc., Kunitachi, Tokyo, Japan.
⁴ Department of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara-Shi, Kanagawa, Japan.
⁵ Regenetiss Inc., Kunitachi, Tokyo, Japan ; Laboratory for Polyphosphate Research, The Kitasato Institute, Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, Japan.

Abstract

Since inorganic polyphosphates [poly(P)] have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of poly(P) on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti-collagen type I antibody but not in those cultured without poly(P). Ultrastructural analysis of cells cultured with poly(P) revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with poly(P), it implies that poly(P) can be an effective material for bone regeneration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / drug effects*
Cell Line
Collagen Type I / metabolism
Cytoplasm / drug effects
Cytoplasm / metabolism
Cytoplasm / ultrastructure
Mice
Microscopy, Electron, Transmission
Microscopy, Phase-Contrast
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / ultrastructure
Osteogenesis / drug effects*
Osteopontin / metabolism
Polyphosphates / pharmacology*
Time Factors
Vacuoles / drug effects
Vacuoles / ultrastructure

Substances

Collagen Type I
Polyphosphates
Osteopontin

Grants and funding

This study was supported in parts by grants from the RegeneTiss Corporation; a Grant-in-Aid for Innovations through Business-Academic-Public Sector Cooperation; a Grant-in-Aid for Scientific Research on Priority Areas (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan; and a Grant-in-Aid for Scientific Research from the Faculty of Health Sciences, Hokkaido University, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.