Abstract
A fluorous tagging strategy coupled with enzymatic synthesis is introduced to efficiently synthesize multiple phosphatidylinositides, which are then directly immobilized on a fluorous polytetrafluoroethylene (PTFE) membrane to probe protein-lipid interactions.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Antibodies / chemistry
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / chemistry
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Phosphatidylinositol 3-Kinases / chemistry
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Phosphatidylinositols / chemical synthesis*
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Phosphatidylinositols / chemistry
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Polytetrafluoroethylene / chemistry
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Protein Array Analysis
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Type C Phospholipases / chemistry
Substances
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Antibodies
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Fluorescent Dyes
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Phosphatidylinositols
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Polytetrafluoroethylene
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Phosphatidylinositol 3-Kinases
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Type C Phospholipases