Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype

J Biol Chem. 2014 Mar 14;289(11):7884-96. doi: 10.1074/jbc.M113.522037. Epub 2014 Feb 3.

Abstract

Glucose is a critical component in the proinflammatory response of macrophages (MΦs). However, the contribution of glucose transporters (GLUTs) and the mechanisms regulating subsequent glucose metabolism in the inflammatory response are not well understood. Because MΦs contribute to obesity-induced inflammation, it is important to understand how substrate metabolism may alter inflammatory function. We report that GLUT1 (SLC2A1) is the primary rate-limiting glucose transporter on proinflammatory-polarized MΦs. Furthermore, in high fat diet-fed rodents, MΦs in crown-like structures and inflammatory loci in adipose and liver, respectively, stain positively for GLUT1. We hypothesized that metabolic reprogramming via increased glucose availability could modulate the MΦ inflammatory response. To increase glucose uptake, we stably overexpressed the GLUT1 transporter in RAW264.7 MΦs (GLUT1-OE MΦs). Cellular bioenergetics analysis, metabolomics, and radiotracer studies demonstrated that GLUT1 overexpression resulted in elevated glucose uptake and metabolism, increased pentose phosphate pathway intermediates, with a complimentary reduction in cellular oxygen consumption rates. Gene expression and proteome profiling analysis revealed that GLUT1-OE MΦs demonstrated a hyperinflammatory state characterized by elevated secretion of inflammatory mediators and that this effect could be blunted by pharmacologic inhibition of glycolysis. Finally, reactive oxygen species production and evidence of oxidative stress were significantly enhanced in GLUT1-OE MΦs; antioxidant treatment blunted the expression of inflammatory mediators such as PAI-1 (plasminogen activator inhibitor 1), suggesting that glucose-mediated oxidative stress was driving the proinflammatory response. Our results indicate that increased utilization of glucose induced a ROS-driven proinflammatory phenotype in MΦs, which may play an integral role in the promotion of obesity-associated insulin resistance.

Keywords: Crown-like Structure; Glucose Transport; Glycolysis; Inflammation; Macrophages; Metabolomics; Mitochondrial Metabolism; Obesity; Pentose Phosphate Pathway.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipose Tissue / metabolism
  • Animals
  • Biological Transport
  • Bone Marrow Cells / cytology
  • Cells, Cultured
  • Female
  • Gene Expression Regulation
  • Glucose / metabolism*
  • Glucose / pharmacokinetics
  • Glucose Transporter Type 1 / metabolism*
  • Immunohistochemistry
  • Inflammation / metabolism*
  • Liver / metabolism
  • Macrophages / cytology*
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / metabolism
  • Pentose Phosphate Pathway
  • Phenotype
  • Proteomics
  • Reactive Oxygen Species / metabolism

Substances

  • Glucose Transporter Type 1
  • Reactive Oxygen Species
  • Slc2a1 protein, mouse
  • Glucose