Abstract
NADPH-P450 reductase (NPR) was previously found to contribute to the hypoxic response of cells, but the mechanism was not clarified. In this study, we identified a cellular stress response (CSR) as a new factor interacting with NPR by a yeast two-hybrid system. Overexpression of CSR enhanced the induction of erythropoietin and hypoxia response element (HRE) activity under hypoxia in human hepatocarcinoma cell lines (Hep3B), while knockdown of CSR suppressed them. This new finding regarding the interaction of NPR with CSR provides insight into the function of NPR in hypoxic response.
Keywords:
Cellular stress response; Hypoxic response; NADPH-P450 reductase.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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Cell Hypoxia
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Cell Line, Tumor
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Erythropoietin / genetics
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Gene Expression Regulation, Neoplastic
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HEK293 Cells
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism*
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Humans
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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NADPH-Ferrihemoprotein Reductase / genetics
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NADPH-Ferrihemoprotein Reductase / metabolism*
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Protein Binding
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RNA Interference
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Response Elements / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Scavenger Receptors, Class A / genetics
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Scavenger Receptors, Class A / metabolism*
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Two-Hybrid System Techniques
Substances
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Heat-Shock Proteins
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SCARA3 protein, human
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Scavenger Receptors, Class A
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Erythropoietin
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NADPH-Ferrihemoprotein Reductase