The pyruvate dehydrogenase complex of Corynebacterium glutamicum: an attractive target for metabolic engineering

J Biotechnol. 2014 Dec 20:192 Pt B:339-45. doi: 10.1016/j.jbiotec.2013.12.019. Epub 2014 Jan 29.

Abstract

The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.

Keywords: Amino and organic acid production; Corynebacterium glutamicum; Isobutanol production; Metabolic engineering; Pyruvate dehydrogenase complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Butanols
  • Corynebacterium glutamicum* / enzymology
  • Corynebacterium glutamicum* / genetics
  • Corynebacterium glutamicum* / metabolism
  • Metabolic Engineering*
  • Pyruvate Dehydrogenase Complex*
  • Pyruvic Acid
  • Valine

Substances

  • Butanols
  • Pyruvate Dehydrogenase Complex
  • isobutyl alcohol
  • Pyruvic Acid
  • Valine