A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus

J Clin Microbiol. 2014 Feb;52(2):663-7. doi: 10.1128/JCM.02191-13. Epub 2013 Dec 4.

Abstract

We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Haemophilus / classification*
  • Haemophilus / genetics*
  • Haemophilus Infections / diagnosis*
  • Haemophilus Infections / microbiology*
  • High-Throughput Screening Assays
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Transition Temperature

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/KF048057
  • GENBANK/KF048058
  • GENBANK/KF048059
  • GENBANK/KF048060
  • GENBANK/KF048061
  • GENBANK/KF048062
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  • GENBANK/KF048067
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  • GENBANK/KF048069
  • GENBANK/KF048070
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  • GENBANK/KF048072
  • GENBANK/KF048073
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  • GENBANK/KF048075
  • GENBANK/KF048076
  • GENBANK/KF048077
  • GENBANK/KF048078
  • GENBANK/KF048079
  • GENBANK/KF048080
  • GENBANK/KF048081
  • GENBANK/KF048082
  • GENBANK/KF048083
  • GENBANK/KF048084
  • GENBANK/KF048085
  • GENBANK/KF048086
  • GENBANK/KF048087