Photobiocatalytic chemistry of oxidoreductases using water as the electron donor

Maria Mifsud; Serena Gargiulo; Sara Iborra; Isabel W C E Arends; Frank Hollmann; Avelino Corma

doi:10.1038/ncomms4145

Photobiocatalytic chemistry of oxidoreductases using water as the electron donor

Nat Commun. 2014:5:3145. doi: 10.1038/ncomms4145.

Authors

Maria Mifsud¹, Serena Gargiulo², Sara Iborra¹, Isabel W C E Arends², Frank Hollmann², Avelino Corma¹

Affiliations

¹ Instituto de Tecnología Química UPV-CSIC, Avda de los Naranjos s/n, Valencia 46022, Spain.
² Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft 2628BL, The Netherlands.

PMID: 24473192
DOI: 10.1038/ncomms4145

Abstract

To date, water has been poorly studied as the sacrificial electron donor for biocatalytic redox reactions using isolated enzymes. Here we demonstrate that water can also be turned into a sacrificial electron donor to promote biocatalytic redox reactions. The thermodynamic driving force required for water oxidation is obtained from UV and visible light by means of simple titanium dioxide-based photocatalysts. The electrons liberated in this process are delivered to an oxidoreductase by simple flavin redox mediators. Overall, the feasibility of photobiocatalytic, water-driven bioredox reactions is demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Electrons*
Kinetics
Oxidoreductases / chemistry
Oxidoreductases / metabolism*
Photochemistry
Thermodynamics
Water / chemistry*

Substances

Water
Oxidoreductases