Abstract
Sortase-mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C-terminal modification of proteins with functional units as well as for the preparation of C-to-C fusion proteins.
Keywords:
hydrazones; hydrolysis; ligation; protein modification; proteins.
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Aminoacyltransferases / metabolism*
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Bacterial Proteins / metabolism*
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Cysteine Endopeptidases / metabolism*
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Fluorescein / chemistry
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Hydrazines / chemistry*
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Hydrolysis
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Peptides / chemistry
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Peptides / metabolism
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism*
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Single-Domain Antibodies / chemistry
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Single-Domain Antibodies / immunology
Substances
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Bacterial Proteins
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Hydrazines
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Peptides
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Recombinant Proteins
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Single-Domain Antibodies
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hydrazine
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Aminoacyltransferases
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sortase A
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Cysteine Endopeptidases
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Fluorescein