Development and application of an immunoaffinity column enzyme immunoassay for mycotoxin zearalenone in complicated samples

Xiaoqian Tang; Xin Li; Peiwu Li; Qi Zhang; Ran Li; Wen Zhang; Xiaoxia Ding; Jiawen Lei; Zhaowei Zhang

doi:10.1371/journal.pone.0085606

Development and application of an immunoaffinity column enzyme immunoassay for mycotoxin zearalenone in complicated samples

PLoS One. 2014 Jan 17;9(1):e85606. doi: 10.1371/journal.pone.0085606. eCollection 2014.

Authors

Xiaoqian Tang¹, Xin Li¹, Peiwu Li², Qi Zhang³, Ran Li³, Wen Zhang³, Xiaoxia Ding⁴, Jiawen Lei¹, Zhaowei Zhang¹

Affiliations

¹ Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China.
² Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China ; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, P. R. China ; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China.
³ Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China ; Key laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China.
⁴ Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China ; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China.

Abstract

The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 µg L(-1). The mAb 2D3 exhibited a high recognition of ZEA (100%) and β-zearalenol (β-ZOL, 88.2%). Its cross-reactivity with α-zearalenol (α-ZOL) and β-zearalanol (β-ZAL) were found to be 4.4% and 4.6%, respectively. The IAC-icELISA method was employed to analyze ZEA contamination in food samples, compared with high-performance liquid chromatography (HPLC). The spiked assay for ZEA demonstrated the considerable recoveries for IAC-icELISA (83-93%) and HPLC (94-108%) methods. Results showed that the mAb 2D3 and IAC-icELISA method posed potential applications in sensitively determination of ZEA in maize.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / metabolism
Breeding
Chromatography, Affinity / methods*
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Female
Hybridomas
Immunoenzyme Techniques / methods*
Mice
Mice, Inbred BALB C
Mycotoxins / analysis*
Zea mays / chemistry
Zeranol / analogs & derivatives*
Zeranol / analysis

Substances

Antibodies
Mycotoxins
zearalenol
Zeranol

Grants and funding

This work was supported by the Project of National Science and Technology Pillar Plan (2012BAB19B09), the Special Fund for Agro-scientific Research in the Public Interest (201203094), The National Natural Science Foundation of China (21205133) and the Key Project of the Ministry of Agriculture (2011-G5). We state the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.