Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site

J Microbiol Methods. 2014 Apr:99:35-7. doi: 10.1016/j.mimet.2014.01.009. Epub 2014 Jan 23.

Abstract

Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensis MR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used.

Keywords: DNA restriction–modification; Shewanella oneidensis; Transformation.

MeSH terms

  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism*
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Plasmids*
  • Sequence Deletion*
  • Shewanella / enzymology*
  • Shewanella / genetics*
  • Transformation, Bacterial*

Substances

  • DNA, Bacterial
  • ATCGAT-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific