Hydroxylamine enhances glucose uptake in C2C12 skeletal muscle cells through the activation of insulin receptor substrate 1

Taro Kimura; Eisuke Kato; Tsukasa Machikawa; Shunsuke Kimura; Shinji Katayama; Jun Kawabata

doi:10.1016/j.bbrc.2014.01.039

Hydroxylamine enhances glucose uptake in C2C12 skeletal muscle cells through the activation of insulin receptor substrate 1

Biochem Biophys Res Commun. 2014 Feb 28;445(1):6-9. doi: 10.1016/j.bbrc.2014.01.039. Epub 2014 Jan 22.

Authors

Taro Kimura¹, Eisuke Kato², Tsukasa Machikawa¹, Shunsuke Kimura¹, Shinji Katayama³, Jun Kawabata¹

Affiliations

¹ Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
² Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. Electronic address: kmra@chem.agr.hokudai.ac.jp.
³ Laboratory of Food Biochemistry, Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.

PMID: 24462868
DOI: 10.1016/j.bbrc.2014.01.039

Abstract

Diabetes mellitus is a global disease, and the number of patients with it is increasing. Of various agents for treatment, those that directly act on muscle are currently attracting attention because muscle is one of the main tissues in the human body, and its metabolism is decreased in type II diabetes. In this study, we found that hydroxylamine (HA) enhances glucose uptake in C2C12 myotubes. Analysis of HA's mechanism revealed the involvement of IRS1, PI3K and Akt that is related to the insulin signaling pathway. Further investigation about the activation mechanism of insulin receptor or IRS1 by HA may provide a way to develop a novel anti-diabetic agent alternating to insulin.

Keywords: Diabetes mellitus; Glucose uptake; Hydroxylamine; Insulin; PI3K/Akt signaling; Skeletal muscle.

MeSH terms

Animals
Blotting, Western
Cell Line
Glucose / pharmacokinetics*
Humans
Hydroxylamine / pharmacology*
Hypoglycemic Agents / pharmacology
Insulin / pharmacology
Insulin Receptor Substrate Proteins / metabolism*
Mice
Muscle Fibers, Skeletal / cytology
Muscle Fibers, Skeletal / drug effects*
Muscle Fibers, Skeletal / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Phosphorylation / drug effects
Proto-Oncogene Proteins c-akt / metabolism
Signal Transduction / drug effects

Substances

Hypoglycemic Agents
Insulin
Insulin Receptor Substrate Proteins
Irs1 protein, mouse
Hydroxylamine
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Glucose