A new dried milk sampling technique and its application for progesterone detection in cows

Jeanne V Samsonova; Alexander P Osipov; Sergey E Kondakov

doi:10.1016/j.tvjl.2013.10.031

A new dried milk sampling technique and its application for progesterone detection in cows

Vet J. 2014 Mar;199(3):471-2. doi: 10.1016/j.tvjl.2013.10.031. Epub 2013 Oct 29.

Authors

Jeanne V Samsonova¹, Alexander P Osipov², Sergey E Kondakov²

Affiliations

¹ Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia. Electronic address: jvs@enz.chem.msu.ru.
² Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.

PMID: 24461204
DOI: 10.1016/j.tvjl.2013.10.031

Abstract

A new method for milk sample collection and storage, based on a dried milk sampling technique, is proposed. The method includes application of a whole milk sample to a porous membrane followed by drying. One hundred whole milk samples (dried and liquid) taken on day 21 post insemination were analysed for progesterone by ELISA and results for both dried and liquid samples were well correlated (r=0.911). Milk progesterone ELISA accuracy for pregnancy diagnosis in cows was 87%.

Keywords: Dried samples; ELISA; Pregnancy diagnosis in cows; Progesterone; Whole milk.

MeSH terms

Animals
Cattle
Enzyme-Linked Immunosorbent Assay / veterinary*
Female
Milk / chemistry*
Pregnancy
Pregnancy, Animal / metabolism*
Progesterone / metabolism*
Sensitivity and Specificity

Substances

Progesterone