Abstract
Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data suggest that the proteins in question are appropriate as a candidate edible vaccine against tuberculosis.
MeSH terms
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Administration, Oral
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Animals
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Antigens, Bacterial / biosynthesis
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Antigens, Bacterial / genetics*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology
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Daucus carota / genetics
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Mice
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / immunology
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Plants, Genetically Modified / genetics*
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Plants, Genetically Modified / growth & development
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Plants, Genetically Modified / immunology
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Tuberculosis / immunology*
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Tuberculosis / prevention & control
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Vaccines, Edible / genetics
Substances
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Antigens, Bacterial
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Bacterial Proteins
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ESAT-6 protein, Mycobacterium tuberculosis
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Vaccines, Edible