APOBEC2 mRNA and protein is predominantly expressed in skeletal and cardiac muscles of chickens

Juan Li; Xiao-Ling Zhao; Elizabeth Ruth Gilbert; Di-Yan Li; Yi-Ping Liu; Yan Wang; Qing Zhu; Ya-Gang Wang; Yang Chen; Kai Tian

doi:10.1016/j.gene.2014.01.003

APOBEC2 mRNA and protein is predominantly expressed in skeletal and cardiac muscles of chickens

Gene. 2014 Apr 15;539(2):263-9. doi: 10.1016/j.gene.2014.01.003. Epub 2014 Jan 15.

Authors

Affiliations

¹ College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China; Research Institute of Raising Livestock, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province 611130, China.
² College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China.
³ Department of Animal and Poultry Sciences 0306, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
⁴ College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, China. Electronic address: zhuqingsicau@163.com.

PMID: 24440786
DOI: 10.1016/j.gene.2014.01.003

Abstract

Apolipoprotein B mRNA-editing enzyme catalytic subunit 2 (APOBEC2) plays an important role in regulating and maintaining muscle development in mammals. In this study, we evaluated APOBEC2 mRNA abundance and protein expression and the results indicated that APOBEC2 mRNA was most abundant in skeletal and cardiac muscle, with relatively low expression in the gonads, gizzard and subcutaneous fat tissues of chickens. Immunoreactive APOBEC2 was localized to the cell nucleus of developing myocardium and skeletal myofibers. There were significant differences in mRNA and protein abundance among ages, tissues, and between males and females. In conclusion, APOBEC2 was expressed as the greatest in skeletal muscle and cardiac muscle where it localized to the nucleus. Thus, APOBEC2 may play an important role in muscle development in chickens.

Keywords: APOBEC2; Chicken; Real-time PCR; Western blotting; mRNA/protein expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

APOBEC-1 Deaminase
Animals
Blotting, Western
Chickens
Cytidine Deaminase / genetics*
Cytidine Deaminase / metabolism*
Female
Gene Expression Regulation / physiology*
Immunoenzyme Techniques
Male
Muscle, Skeletal / metabolism*
Myocardium / metabolism*
RNA, Messenger / genetics*
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction

Substances

RNA, Messenger
APOBEC-1 Deaminase
Cytidine Deaminase