Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade

A Revoir; D J Ballard; D Syndercombe Court

doi:10.1016/j.scijus.2013.08.005

Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade

Sci Justice. 2014 Jan;54(1):95-7. doi: 10.1016/j.scijus.2013.08.005. Epub 2013 Sep 7.

Authors

A Revoir¹, D J Ballard², D Syndercombe Court²

Affiliations

¹ King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK. Electronic address: andrew.revoir@kcl.ac.uk.
² King's College London, Department of Forensic and Analytical Science, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.

PMID: 24438784
DOI: 10.1016/j.scijus.2013.08.005

Abstract

Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.

Keywords: D16S539; Discordance; Multiplex Upgrade Project; Next generation multiplex kits; UK National DNA database.

MeSH terms

DNA Fingerprinting / instrumentation*
Databases, Genetic
Humans
Microsatellite Repeats*
Multiplex Polymerase Chain Reaction
Sequence Analysis, DNA