Abstract
A new method is proposed which combines the high spatial resolution of transmission electron microscopy with information on the dynamics of transcription. Incorporation of two different RNA precursors was used to define a time transcription window on cultured cells treated with hypometabolizing peptides which are known to modulate transcription. This procedure allows detecting a single fibril of newly synthesized RNA in the time range in which it is transcribed.
MeSH terms
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Cell Line, Tumor
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Cell Nucleus
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Cell Proliferation
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Dactinomycin / pharmacology
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Dichlororibofuranosylbenzimidazole / pharmacology
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Enkephalin, Leucine-2-Alanine / analogs & derivatives
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Enkephalin, Leucine-2-Alanine / pharmacology*
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HeLa Cells
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Humans
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Immunohistochemistry / methods
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Microscopy, Electron, Transmission / methods
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RNA Precursors / chemistry*
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RNA Precursors / genetics
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RNA, Ribosomal / biosynthesis
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RNA, Ribosomal / genetics*
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Staining and Labeling / methods
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Transcription, Genetic / drug effects
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Transcription, Genetic / genetics*
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Uridine / analogs & derivatives
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Uridine / chemistry
Substances
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RNA Precursors
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RNA, Ribosomal
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Dactinomycin
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Dichlororibofuranosylbenzimidazole
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Enkephalin, Leucine-2-Alanine
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Uridine