Defatted wheat germ peptides (DWGPs) were prepared by fermentation with Bacillus Subtilis B1 and the antioxidant activities of DWGPs were investigated. The fermentation condition was optimized by response surface method (RSM) with three factors and three levels according to Box-Behnken theory. A maximal yield of DWGPs was achieved 8.69 mg/mL under optimal conditions: inoculum size 8%, fermentation temperature 31 °C and time 48 h. The main portion in the hydrolysates after fermentation was not free amino acid but peptide. The main molecular weight distribution of DWGPs was lower than 1000 Da. A positive correlation (R(2) = 0.9911) was found between concentration of DWGPs and total antioxidant capacity (T-AOC). DWGPs presented a significant does-dependent on scavenging activities of DPPH, hydroxyl and superoxide anion radicals. The EC50 values for the scavenging rates of DPPH, hydroxyl and superoxide anion radicals were 3.16 mg/mL, 6.04 mg/mL and 7.46 mg/mL, respectively. The results suggested that DWGPs produced by fermentation could be used as a promising antioxidant ingredient.
Keywords: Bacillus Subtilis B1; Defatted wheat germ peptides; Fermentation; Free radical scavenging activity.