Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules

Virology. 2014 Jan 20:449:109-19. doi: 10.1016/j.virol.2013.11.006. Epub 2013 Nov 28.

Abstract

The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, it is unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement of Gag but not Env at 4h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles.

Keywords: Anterograde transport; Cytoskeleton; Envelope; Gag; Live cell-imaging; M-PMV.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Membrane / virology
  • Chlorocebus aethiops
  • Gene Products, env / genetics
  • Gene Products, env / metabolism*
  • Gene Products, gag / genetics
  • Gene Products, gag / metabolism*
  • Macaca mulatta
  • Mason-Pfizer monkey virus / genetics
  • Mason-Pfizer monkey virus / metabolism*
  • Microtubules / metabolism
  • Microtubules / virology*
  • Protein Transport
  • Simian Acquired Immunodeficiency Syndrome / metabolism
  • Simian Acquired Immunodeficiency Syndrome / virology*

Substances

  • Gene Products, env
  • Gene Products, gag