Steady increment of immature platelet fraction is suppressed by irradiation in single-donor platelet components during storage

PLoS One. 2014 Jan 8;9(1):e85465. doi: 10.1371/journal.pone.0085465. eCollection 2014.

Abstract

Circulating immature platelet fraction (IPF) reflects real-time thrombopoiesis and correlates with platelet recovery from thrombocytopenic presentations. To understand the dynamics of IPF in platelet transfusions, we quantified the %-IPF in single-donor platelet components (SDP) during prolonged storage. %-IPF significantly increased from baseline by day 5 post-donation. Absolute IPF counts (A-IPC) had similar significant increments. However, gamma-irradiation suppressed the increments of %-IPF and A-IPC by >50%. Ultrastructural analysis of SDP units at day 10 showed well preserved morphology of immature platelets. Our findings suggest that IPF might actively expand ex-vivo and may have a longer shelf life than their mature counterparts. Closer study of IPF may be of critical clinical importance for transfusion practices.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Platelets / radiation effects*
  • Blood Platelets / ultrastructure
  • Cell Proliferation / radiation effects*
  • Cells, Cultured
  • Gamma Rays
  • Humans
  • Microscopy, Electron, Transmission
  • Platelet Count
  • Platelet Transfusion
  • Temperature
  • Thrombocytopenia / pathology
  • Thrombocytopenia / therapy
  • Thrombopoiesis / physiology
  • Time Factors

Grants and funding

This study was fully supported by a grant from the Office of Diversity and Inclusion of the University Hospitals Case Medical Center, Cleveland, Ohio, awarded to RWM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.