Development of microsatellite-enriched libraries

Hélène Vignes; Ronan Rivallan

doi:10.1007/978-1-62703-767-9_9

Development of microsatellite-enriched libraries

Methods Mol Biol. 2014:1115:177-89. doi: 10.1007/978-1-62703-767-9_9.

Authors

Hélène Vignes¹, Ronan Rivallan

Affiliation

¹ Cirad UMR AGAP, Montpellier, France.

PMID: 24415475
DOI: 10.1007/978-1-62703-767-9_9

Abstract

Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.

MeSH terms

Cloning, Molecular
DNA, Plant / genetics
Dinucleotide Repeats / genetics
Genetic Loci / genetics
Genome, Plant / genetics
Genomic Library*
Microsatellite Repeats / genetics*
Oligonucleotide Probes / genetics
Plasmids / genetics
Polymerase Chain Reaction
Sequence Analysis, DNA
Transformation, Genetic

Substances

DNA, Plant
Oligonucleotide Probes