Short communication: Detection of non-culturable Vibrio cholerae O139, by PCR and fluorescent antibody methods, in laboratory microcosms

M S Islam; M J Alam; M A Miah; A Felsenstein; R B Sack

doi:10.1007/BF00286382

Short communication: Detection of non-culturable Vibrio cholerae O139, by PCR and fluorescent antibody methods, in laboratory microcosms

World J Microbiol Biotechnol. 1995 Sep;11(5):597-8. doi: 10.1007/BF00286382.

Authors

M S Islam¹, M J Alam, M A Miah, A Felsenstein, R B Sack

Affiliation

¹ Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), GPO Box 128, 1000, Dhaka, Bangladesh.

PMID: 24414922
DOI: 10.1007/BF00286382

Abstract

Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks.