Development of amperometric L-tyrosine sensor based on Fe-doped hydroxyapatite nanoparticles

Mater Sci Eng C Mater Biol Appl. 2014 Feb 1:35:85-91. doi: 10.1016/j.msec.2013.10.013. Epub 2013 Nov 1.

Abstract

A novel biosensor based on Fe-doped hydroxyapatite (Fe-HA) nanoparticles and tyrosinase has been developed for the detection of L-tyrosine. Nanostructured Fe-HA was synthesized by a simple microwave irradiation method, and its phase formation, morphology and magnetic property were examined by powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Electrochemical performance of the nano Fe-HA/tyrosinase modified glassy carbon electrode (GCE) for detection of L-tyrosine was investigated by cyclic voltammetry (CV) and amperometric methods. The fabricated biosensor exhibited a linear response to L-tyrosine over a wide concentration range of 1.0×10(-7) to 1.0×10(-5) M with a detection limit of 245 nM at pH 7.0. In addition, the fabricated sensor showed an excellent selectivity, good reproducibility, long-term stability and anti-interference towards the determination of L-tyrosine.

Keywords: Fe-doped hydroxyapatite; biosensor; direct electrochemistry; l-tyrosine; tyrosinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation
  • Conductometry / instrumentation*
  • Durapatite / chemistry*
  • Electrodes*
  • Equipment Design
  • Equipment Failure Analysis
  • Iron / chemistry*
  • Monophenol Monooxygenase / chemistry*
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Nanotechnology / instrumentation
  • Tyrosine / analysis*
  • Tyrosine / chemistry

Substances

  • Tyrosine
  • Durapatite
  • Iron
  • Monophenol Monooxygenase