Discovery of tumor markers for gastric cancer by proteomics

Jeng-Yih Wu; Chun-Chia Cheng; Jaw-Yuan Wang; Deng-Chyang Wu; Jan-Sing Hsieh; Shui-Cheng Lee; Wen-Ming Wang

doi:10.1371/journal.pone.0084158

Discovery of tumor markers for gastric cancer by proteomics

PLoS One. 2014 Jan 3;9(1):e84158. doi: 10.1371/journal.pone.0084158. eCollection 2014.

Authors

Jeng-Yih Wu¹, Chun-Chia Cheng², Jaw-Yuan Wang³, Deng-Chyang Wu⁴, Jan-Sing Hsieh³, Shui-Cheng Lee⁵, Wen-Ming Wang¹

Affiliations

¹ Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan ; Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan ; Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
² Institute of Nuclear Energy Research, Atomic Energy Council, Executive Yuan, Taiwan.
³ Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung,Taiwan.
⁴ Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan ; Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan ; Department of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁵ Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Abstract

Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered.This study intended to directly discover differential biomarkers from GC tissues by two-dimension-differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC).Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG.Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions.This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Aged, 80 and over
Biomarkers, Tumor / metabolism*
Endoplasmic Reticulum Chaperone BiP
Female
Humans
Immunohistochemistry
Male
Middle Aged
Neoplasm Staging
Proteomics* / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach Neoplasms / diagnosis
Stomach Neoplasms / metabolism*
Stomach Neoplasms / mortality
Two-Dimensional Difference Gel Electrophoresis

Substances

Biomarkers, Tumor
Endoplasmic Reticulum Chaperone BiP
HSPA5 protein, human

Grants and funding

This work was supported by grants NSC96-3111-P-042A-004-Y, NSC100-2314-B-037-040, and by National Science Council of Republic of China. This work was also supported by Kaohsiung Medical University Hospital (KMUH97-7R32, KMUH98-8R33). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.