Multiple enzymatic profiles of Vibrio parahaemolyticus strains isolated from oysters

Renata Albuquerque Costa; Ludimila M M Conde Amorim; Rayza Lima Araújo; Regine H Silva dos Fernandes Vieira

doi:10.1016/S0325-7541(13)70035-X

Multiple enzymatic profiles of Vibrio parahaemolyticus strains isolated from oysters

Rev Argent Microbiol. 2013 Oct-Dec;45(4):267-70. doi: 10.1016/S0325-7541(13)70035-X.

Authors

Renata Albuquerque Costa¹, Ludimila M M Conde Amorim², Rayza Lima Araújo², Regine H Silva dos Fernandes Vieira²

Affiliations

¹ Sea Sciences Institute, Federal University of Ceará, Abolição 3207, 60165-081, Fortaleza, Brazil. Electronic address: renata.albuq@gmail.com.
² Sea Sciences Institute, Federal University of Ceará, Abolição 3207, 60165-081, Fortaleza, Brazil.

PMID: 24401782
DOI: 10.1016/S0325-7541(13)70035-X

Abstract

The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presence of gelatinase (GEL), caseinase (CAS), elastase (ELAS), phospholipase (PHOS), lipase (LIP), amilase (AML) and DNase. All enzymes, except elastase, were detected in more than 60% of the strains. The most recurrent enzymatic profiles were AML + DNase + PHOS + GEL + LIP (n=16; 22.9%) and AML + CAS + DNase + PHOS + GEL + LIP (n=21; 30%). Considering the fact that exoenzyme production by vibrios is closely related to virulence, one must be aware of the bacteriological risk posed to human health by the consumption of raw or undercooked oysters.

Keywords: Bivalves; Bivalvos; Brasil; Brazil; Exoenzimas; Exoenzymes; Vibrio parahaemolyticus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Crassostrea / microbiology*
Vibrio parahaemolyticus / classification*
Vibrio parahaemolyticus / enzymology*
Vibrio parahaemolyticus / isolation & purification