Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Methods Mol Biol. 2014:1116:209-34. doi: 10.1007/978-1-62703-764-8_15.

Abstract

In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular / methods*
  • DNA Primers / genetics
  • Escherichia coli / genetics*
  • Genetic Vectors / genetics*
  • Polymerase Chain Reaction
  • Transformation, Genetic

Substances

  • DNA Primers