Co-localization of L-type voltage dependent calcium channel alpha 1D subunit (Ca(v)1.3) and calbindin (CB) in the mouse central nervous system

Abstract

Previous study has shown that the co-localization of calbindin (CB) with L-type voltage dependent Ca(2+) channel (VDCC) alpha 1C subunit (Ca(v)1.2) in the rat insulinoma 1046-38 (RIN) beta cells may play an important regulatory role in Ca(2+) influx and exocytosis of insulin granules. In the present study, L-type voltage dependent Ca(2+) channel (VDCC) and calbindin (CB) were demonstrated in different regions of the mouse central nervous system (CNS). Double labeling immunofluorescence staining showed a co-localization of Ca(v)1.3 and CB. The co-localization of Ca(v)1.3 and CB in certain brain regions such as the hippocampus suggests their important roles in neuroplasticity. The relative high percentages of co-localization of Ca(v)1.3 with CB in the laminae II of the dorsal horn of the spinal cord indicate that the regulation mechanism of nociceptive transmission may be related with both VDCC and Ca(2+) binding protein.

Keywords: CB; Ca(v)1.3; Central nervous system; Co-localization.