Abstract
The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / immunology
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Cell Membrane / genetics
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Cell Membrane / metabolism
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Endocytosis / genetics*
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Endosomal Sorting Complexes Required for Transport / genetics*
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Endosomal Sorting Complexes Required for Transport / metabolism
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Endosomes / genetics
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Endosomes / metabolism
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Plant Immunity / genetics*
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Protein Kinases / genetics*
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Protein Kinases / metabolism
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rab5 GTP-Binding Proteins / genetics
Substances
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Arabidopsis Proteins
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Endosomal Sorting Complexes Required for Transport
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Protein Kinases
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FLS2 protein, Arabidopsis
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rab5 GTP-Binding Proteins
Grants and funding
TS and MB were supported by a grant of the Deutsche Forschungsgemeinschaft (SPP1212; postdoctoral fellow), SSa by a fellowship of the Max-Planck-Institute for Plant Breeding Research (Cologne), and research by SR is supported by the Gatsby Charitable Foundation and a grant by the European Research Council (ERC STORM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.