Highly emissive deoxyguanosine analogue capable of direct visualization of B-Z transition

Chem Commun (Camb). 2014 Feb 14;50(13):1573-5. doi: 10.1039/c3cc48297a.

Abstract

A 2-aminothieno[3,4-d]pyrimidine G-mimic deoxyribonucleoside, (th)dG, was synthesized and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue. We demonstrate that (th)dG enables the visual detection of Z-DNA successfully based on different π-stacking of B- and Z-DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, B-Form / analysis*
  • DNA, Z-Form / analysis*
  • Deoxyguanosine / analogs & derivatives*
  • Fluorescent Dyes / chemistry*
  • Oligonucleotides / chemistry*

Substances

  • DNA, B-Form
  • DNA, Z-Form
  • Fluorescent Dyes
  • Oligonucleotides
  • Deoxyguanosine