Trx2p-dependent regulation of Saccharomyces cerevisiae oxidative stress response by the Skn7p transcription factor under respiring conditions

PLoS One. 2013 Dec 23;8(12):e85404. doi: 10.1371/journal.pone.0085404. eCollection 2013.

Abstract

The whole genome analysis has demonstrated that wine yeasts undergo changes in promoter regions and variations in gene copy number, which make them different to lab strains and help them better adapt to stressful conditions during winemaking, where oxidative stress plays a critical role. Since cytoplasmic thioredoxin II, a small protein with thiol-disulphide oxidoreductase activity, has been seen to perform important functions under biomass propagation conditions of wine yeasts, we studied the involvement of Trx2p in the molecular regulation of the oxidative stress transcriptional response on these strains. In this study, we analyzed the expression levels of several oxidative stress-related genes regulated by either Yap1p or the co-operation between Yap1p and Skn7p. The results revealed a lowered expression for all the tested Skn7p dependent genes in a Trx2p-deficient strain and that Trx2p is essential for the oxidative stress response during respiratory metabolism in wine yeast. Additionally, activity of Yap1p and Skn7p dependent promoters by β-galactosidase assays clearly demonstrated that Skn7p-dependent promoter activation is affected by TRX2 gene deficiency. Finally we showed that deleting the TRX2 gene causes Skn7p hyperphosphorylation under oxidative stress conditions. We propose Trx2p to be a new positive efector in the regulation of the Skn7p transcription factor that controls phosphorylation events and, therefore, modulates the oxidative stress response in yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Catalase / metabolism
  • DNA Primers / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Deletion
  • Gene Expression Regulation, Fungal / physiology*
  • Immunoprecipitation
  • Molecular Sequence Data
  • Oxidative Stress / physiology*
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Thioredoxins / genetics
  • Thioredoxins / metabolism*
  • Transcription Factors / metabolism*
  • Wine / microbiology*
  • beta-Galactosidase

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • SKN7 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TRX2 protein, S cerevisiae
  • Transcription Factors
  • YAP1 protein, S cerevisiae
  • Thioredoxins
  • Catalase
  • beta-Galactosidase

Grants and funding

This work has been supported by grants AGL 2008-00060 and AGL2011-24353 from the Spanish Ministry of Education and Science (MEC) to EM. EG was a predoctoral fellow of the FPI program from the MEC (Spanish Ministry of Education). RG-P was a predoctoral fellow of the I3P program from the CSIC (Spanish National Research Council). RP-T was a postdoctoral fellow of the JAEDOC program from the CSIC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.