PVv3, a new shuttle vector for gene expression in Vibrio vulnificus

Appl Environ Microbiol. 2014 Feb;80(4):1477-81. doi: 10.1128/AEM.03720-13. Epub 2013 Dec 20.

Abstract

An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Electroporation / methods
  • Escherichia coli / genetics*
  • Gene Expression*
  • Genes, Reporter
  • Genetic Vectors*
  • Genomic Instability
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / genetics
  • Molecular Biology / methods*
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Transformation, Bacterial
  • Vibrio cholerae / genetics
  • Vibrio parahaemolyticus / genetics
  • Vibrio vulnificus / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • VvhA protein, Vibrio vulnificus
  • Green Fluorescent Proteins

Associated data

  • GENBANK/HG326273