The identification and pharmacokinetic studies of metabolites of salvianolic acid B after intravenous administration in rats

Chin J Nat Med. 2013 Sep;11(5):560-5. doi: 10.1016/S1875-5364(13)60101-6.

Abstract

Aim: To identify and quantify the major metabolites of salvianolic acid B (SAB) after intravenous injection in rats.

Methods: LC-IT/TOF-MS was used to identify the metabolites in rat bile, plasma, and urine; LC-MS/MS was used to quantify the two major metabolites.

Results: In rat bile, plasma, and urine, nine metabolites were identified, including methylated metabolites of SAB, lithospermic acid (LSA), the decarboxylation and methylation metabolites of LSA, salvianolic acid S (SAS), and dehydrated-SAS. The t1/2 of monomethyl-SAB and LSA were both very short, and monomethyl-SAB had a larger AUC than LSA in rats.

Conclusion: Nine metabolites were found, the metabolic pathway was described, and the pharmacokinetic profiles of LSA and monomethyl-SAB were studied, thereby clarifying that methylation was the dominant metabolic pathway for SAB in rats.

Keywords: Identification; Metabolites; Pharmacokinetics; Salvianolic acid B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravenous
  • Animals
  • Benzofurans / administration & dosage
  • Benzofurans / chemistry
  • Benzofurans / metabolism*
  • Benzofurans / pharmacokinetics*
  • Bile / chemistry
  • Male
  • Molecular Structure
  • Plasma / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Urine / chemistry

Substances

  • Benzofurans
  • salvianolic acid B