Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

Bobby L Boyanton Jr; Amro Almradi; Mark J Espy; Anne E Prada; John P Gibson; Bobbi S Pritt

doi:10.1128/JCM.03062-13

Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves

J Clin Microbiol. 2014 Mar;52(3):957-9. doi: 10.1128/JCM.03062-13. Epub 2013 Dec 18.

Authors

Bobby L Boyanton Jr¹, Amro Almradi, Mark J Espy, Anne E Prada, John P Gibson, Bobbi S Pritt

Affiliation

¹ Department of Clinical Pathology, Beaumont Health System, Royal Oak, Michigan, USA.

Abstract

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

Publication types

Case Reports

MeSH terms

Female
Herpes Simplex / diagnosis*
Herpes Simplex / virology*
Herpesvirus 1, Human / classification
Herpesvirus 1, Human / genetics
Herpesvirus 1, Human / isolation & purification*
Herpesvirus 2, Human / classification
Herpesvirus 2, Human / genetics
Herpesvirus 2, Human / isolation & purification*
Humans
Molecular Diagnostic Techniques / methods*
Polymorphism, Genetic
Real-Time Polymerase Chain Reaction / methods*
Transition Temperature
Virology / methods*
Young Adult