The Panhandle formed by influenza A and C virus NS non-coding regions determines NS segment expression

Bernadette Crescenzo-Chaigne; Cyril Barbezange; Sylvie van der Werf

doi:10.1371/journal.pone.0081550

The Panhandle formed by influenza A and C virus NS non-coding regions determines NS segment expression

PLoS One. 2013 Nov 21;8(11):e81550. doi: 10.1371/journal.pone.0081550. eCollection 2013.

Authors

Bernadette Crescenzo-Chaigne¹, Cyril Barbezange¹, Sylvie van der Werf¹

Affiliation

¹ Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France ; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France ; Unité de Génétique Moléculaire des Virus à ARN, Université Paris Diderot Sorbonne Paris Cité, Paris, France.

Abstract

Exchange of the extremities of the NS segment of type A and C influenza viruses in reverse genetics systems was used to assess their putative role in type specificity. Restoration of each specific proximal panhandle was mandatory to allow the rescue of viruses with heterotypic extremities. Moreover, the transcription level of the modified segment seemed to be directly affected by the distal panhandle strength.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gammainfluenzavirus / genetics
Gammainfluenzavirus / metabolism*
Humans
Influenza A virus / genetics
Influenza A virus / metabolism*
Viral Nonstructural Proteins / chemistry*
Viral Nonstructural Proteins / genetics
Viral Nonstructural Proteins / metabolism*

Substances

Viral Nonstructural Proteins

Grants and funding

This work was supported by institutional funds from the Institut Pasteur, the Centre National de la Recherche Scientifique and the University Paris Diderot, Sorbonne Paris Cité, Paris, France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.